Tutorial    Troubleshooting    Downloads

Getting an NMR Account

Schedule an appointment with facility manager for initial training

Setup practice session and train with facility manager until acceptable proficiency is obtained

Review NMR Policies

Review NMR Training Policies

Review and Sign NMR User Agreement


Tutorials

Abbreviations and Terms

NMR Sample Preparation

NMR Traing for the 500MHz NMR (Getting started, 1D 1H, 1D 13C and DEPT Experiments)

1D NMR Quick Guide for VNMR 6.1C

NMR of Other Nuclei

            19F 1D Experiment on 500 MHz NMR (Nightmare)

            11B 1D Experiment on 500 MHz NMR (NMR500)

NMR Training for the 600 MHz NMR with Chempack (1H 90-degree Pulse Calibration)

NMR 600 MHz BioPack Training (water suppression, 1H and 15N 90-degree Pulse Calibration)

Advance Chempack Experiments

    1D NMR Experiments

            Pure Shift 1D (PS1D.c)

            NOESY 1D (NOESY1D.c)

            ROESY 1D (ROESEY1D.c)

            TOCSY 1D (TOCSY1D.c)

            selexcit (selexcit.c)

            stepNOESY1D (stepNOESY1D.c)

    Homonuclear 2D

            COSY (COSY.c and gCOSY.c)

            DQCOSY (DQCOSY.c and gDQCOSY.c)

            TOCSY

            NOESY   

            ROESY

    Heteronuclear 2D 1-Bond - J1(CH) Correlation

            HSQCAD (HSQCAD.c and gHSQCAD.c)

            HSQC (HSQC.c and gHSQC.c)

            c2hsqcse (c2hsqcse.c and gc2hsqcse.c)

            HMQC (HMQC.c and gHMQC.c)

    Heteronuclear 2D Multiple-Bond - Jn(CH) Correlation

            gHMBCAD (gHMBCAD.c)

            gc2hmbc (gc2hmbc.c)

            gH2BCAD (gH2BCAD.c)

            gc2h2bc (gc2h2bc.c)

    Hetero-TOCSY - (HC)HetToxys

            HSQCADTOXY (HSQCADTOXY.c and gHSQCADTOXY.c)

            HSQCTOXY (HSQCTOXY.c and gHSQCTOXY.c)

    Selective 2D - Sel2D

            (HC)bsHSQCAD (bsHSQCAD and gbsHSQCAD)

            (HC)bsgHMBC (bsgHMBC.c)

            (HC)EXSIDE (EXSIDE.c) *** Better version of J-HMBC for long range HC-coupling ***

            (HC)bgHSQCNOESY (bgHSQCNOESY.c)

            (HC)bsHSQCROESY (bgHSQCROESY)

    Non-Uniform Sampling (NUS) for Chempack           

Advance BioPack Experiment

    Protein Backbone Assignment NH-Detected Experiments

            HNCO (ghn_co.c)

            HN(CO)CA (ghn_co_ca.c)

            HN(CA)CO (ghn_ca_co.c)

            (HCA)CO(CA)NNH (ghca_co_canh.c)

            HNCACB (ghn_cacb.c)

            HBHA(CO)NH (ghbha_co_nh.c)

            CBCA(CO)NNH (gcbca_co_nh.c)

            H(CCO)NH (ghc_co_nh.c)

            C(CO)NH (gc_co_nh.c)

            HNCA (ghn_ca.c)

    Protein Sidechain Assignment CH-Detected Experiments

            HCCHTOCSY (hcch_tocsy.c)

            CB(CGCDCD)He (hbcbcgcdceheA.c)

            CB(CGCD)HD (hbcbcgcdhdA.c)    

    Common 2D Indirect Detection Experiments

            N15 HSQC (gNhsqc.c)

            N15 T1 (gNhsqc.c)

            N15 T2 (gNhsqc.c)

            N15 Watergate HSQC (gNfhsqc.c)

            N15 HSQC-TROSY (gNhsqcTROSY.c)

            N15 DD/CSA Cross Relaxation 9sSCT_CCR.c)

            C13 HSQC (gChsqc.c) *** All Carbons, Aliphatic, Aromatic, Constant Time ***

    C13/N15 Coupling Measurements

            NH 1 Bond Coupling N15 J-Modulation

            NH 1 Bond Coupling N15 HSQC-TROSY (gNhsqc.c)

            CO-Filtered jnco/Dnco (COfiltered-TROSYnietl_sCT.c)

            Ca-Filtered jnca/Dca (Cafiltered_TROSYnietl_sCT.c)  

    Common 3D Indirect Detection Experiments

            N15 TOCSYHSQC (gtocsyNhsqc.c)

            N15 NOESYHSQC (gnoesyNhsqc.c)

            C13 NOESYHSQC Aliphatic (gnoesyChsqc.c)

            C13 NOESYHSQC Aromatic (gnoesyChsqc.c)

NMR Data Processing

    VnmrJ

            Processing 1D NMR Data

            Processing 2D NMR Data

    NMRPipe

            Processing 2D NMR Data

            Processing 3D NMR Data

    MestReNova

            Processing 1D NMR Data

            Processing 2D NMR Data

NMR Equipments

Access NMR Data

Glossary of VnmrJ Commands

Useful Linux Commands


Troubleshooting

   Problems with LOCK

    Problems with SHIMMING

    Problems with CALIBRATING 90-DEGREE PULSE WIDTH


Downloads (Password Protected)

    MestReNova

            Version 9.1.0 for Windows

            Version 9.1.0 for MAC

            UCSC MestReNova License File

            User Manual for 9.1.0

    NMRPipe

            All Version


Agilent NMR Spectrometer Operation Manual (Password Protected)

VnmrJ 4.2 User Guide

VnmrJ 4.2 User Programming

VnmrJ 4.2 Chempack Experiment Guide

VnmrJ 4.2 BioPack User Guide

VnmrJ 4.2 BioPack Protein Backbone Experiment Guide

VnmrJ 4.2 BioPack Protein C13/N15 Indirect Detection Experiment Guide

VnmrJ 4.2 BioPack 1D Homonuclear Experiment Guide

VnmrJ 4.2 BioPack 2D Homonuclear Experiment Guide

VnmrJ 4.2 BioPack RNA/DNA Experiment Guide